a10657-01 Hamamatsu Photonics, K.K.,, a10657-01 Datasheet - Page 2

• Manufacturer Part Number: a10657-01
• Description: Microwell Slide
• Manufacturer: Hamamatsu Photonics, K.K.,

HAMAMATSU PHOTONICS K.K., Electron Tube Division
314-5, Shimokanzo, Iwata City, Shizuoka Pref., 438-0193, Japan, Telephone: [phone], Fax: [phone]
IMeasurement example 1: EGFP expressing and non-expressing HeLa cell stained
IMeasurement example 2: FCS measurement in HeLa cell
Courtesy of Mr. Keishi Sakata, Ms. Makiyo Uchida, Prof. Masataka Kinjo, Laboratory of Molecular Cell Dynamics,
Faculty of Advanced Life Science, Graduate School of Life Science, Hokkaido University
U.S.A.: Hamamatsu Corporation: 360 Foothill Road, P. O. Box 6910, Bridgewater. N.J. 08807-0910, U.S.A., Telephone: [phone], Fax: [phone] E-mail: [e-mail]
Germany: Hamamatsu Photonics Deutschland GmbH: Arzbergerstr. 10, D-82211 Herrsching am Ammersee, Germany, Telephone: [phone], Fax: [phone] E-mail: [e-mail]
France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, 91882 Massy Cedex, France, Telephone: [phone], Fax: [phone] E-mail: [e-mail]
United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court, 10 Tewin Road Welwyn Garden City Hertfordshire AL7 1BW, United Kingdom, Telephone: [phone], Fax: [phone] E-mail: [e-mail]
North Europe: Hamamatsu Photonics Norden AB: Smidesvägen 12, SE-171-41 SOLNA, Sweden, Telephone: [phone], Fax: [phone] E-mail: [e-mail]
Italy: Hamamatsu Photonics Italia: S.R.L.: Strada della Moia, 1/E, 20020 Arese, (Milano), Italy, Telephone: [phone], Fax: [phone] E-mail: [e-mail]
MICROWELL SLIDE A10657-01
MEASUREMENT EXAMPLES
1.010
1.005
1.000
4.5
5
4
10
0
with MitoTracker Deep Red 633 FM
0
10
5
2
Time (s)
τ (µs)
WEB SITE www.hamamatsu.com
• System: Confocal laser scanning microscope
• Sample: HeLa cell (EGFP expressing and non-expressing)
• Orthochromatic dye: MitoTracker Deep Red 633 FM
• Staining conditions: 100 nM in OPTI-MEM, 37 °C, 5 %CO
Laser
He-Ne 633 nm 3 %
Ar 488 nm 1 %
10
10
4
NFT 635 VIS
LP 650
15
10
6
• System: Confocal laser scanning microscope
• Sample: EGFP expressing HeLa cell
• Excitation light: Ar
• Fluorescence filter: 500 nm to 550 nm
• Measurement time: 15 s × 5 times
(Molecular Probes, M22426)
(Ex 644 nm, Em 665 nm)
HFT UV/488/543/633
C-Apo 40 × /1.2 W
BP 500-550 IR
(cell cytoplasm)
HFT: Main dichroic beam spliter
NFT: Secondary dichroic beam spliter
BP : Band pass filter
LP : Long pass filter
+
laser: 488 nm FM
2
, 20 min
TOTH1004E03
APR. 2009 IP